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Mix gently. Centrifuge at 1,200 rpm for 5 minutes to pellet cells. Discard media and resuspend pellet gently in 10 ml warm medium. Divide cells into two T-25 flasks containing 5 ml warm media. Place in incubator. After two days, remove the media and add fresh media. When cells are 6-8x105 cells/ml, split them 1:4 with fresh media. . Analegg

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2.2.3 Infection and immunity. The Jurkat cell line is an immortalized T lymphocyte cell line that was originally obtained from the peripheral blood of a boy with T cell leukemia [14 ]. The Jurkat cell line has most often been used as a prototypical T cell line to study multiple events in T cell biology, including a) T cell signaling and b ... . Livejasminn

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